Single-cell transcriptomic analysis has provided an unprecedented avenue for the identification of neuronal subtypes that are affected in neurological diseases.However, this strategy is largely hindered by the difficulty of collecting fresh samples of adult human brain.Instead,postmortem brain tissue from both normal and diseased human subjects is more accessible.To reduce sample contamination from other ceils or RNA degradation often encountered in whole-cell dissociation and isolation,sequencing of RNA from single nuclei has been developed to reflect whole-cell RNA levels in the human brain [1, 2].By using single-nucleus RNA sequencing (snRNA-seq),recent studies have identified cell-type-specific molecular changes in the neocortex of patients with autism and Alzheimer\'s disease [3-5].