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本文目的是研究噪声对实验动物自然免疫指标的影响。实验采用37只雄性大鼠,每天接触65 dBA噪声5小时,一周5天,共2个月。结束时处死9只测血清自然免疫指标:杀菌活性,溶菌酶,β-溶素,正常补体和抗组织补体结合性自身抗体。另9只动物腹腔一次兔疫接种含Vi抗原的乙醇单价伤寒菌苗,分别在接种后7天、14天、21天自尾静脉取血测抗伤寒菌O、Vi抗体滴度。同时测对照动物。血清杀菌活性测定方法:将培养24小时的大肠菌或葡萄球菌分别接种到用肉汤稀释10倍的受检血清和肉汤中(对照),37℃培养3小
The purpose of this paper is to study the effects of noise on natural immune indices in laboratory animals. A total of 37 male rats were exposed to 65 dBA of noise for 5 hours daily for 5 days a week for 2 months. At the end of the test, 9 serums were sacrificed for natural immunity: bactericidal activity, lysozyme, β-lysin, normal complement and anti-tissue complement-associated autoantibodies. The other 9 animals were inoculated intraperitoneally once a week with monovalent hepatitis E virus-containing monovalent typhoid bacillus vaccine. Anti-typhoid O and Vi antibody titers were obtained from the tail vein at 7 days, 14 days and 21 days after inoculation. At the same time control animals. Method for measuring serum bactericidal activity: Escherichia coli or staphylococcus cultured for 24 hours were respectively inoculated into test sera and broth diluted 10 times with broth (control), and cultured at 37 ° C for 3 hours