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在15℃、相同氮负荷和添加强度条件下运行SBR和CSTR以对比分析两种典型流态(推流式和完全混合式)对硝化菌添加强化硝化效果的影响.结果表明,添加期间,SBR中氨氧化速率(AUR)和亚硝酸盐氧化速率(NUR)分别为添加前的2.34和2.39倍,停止添加后又分别降为添加前的2.01和1.78倍;添加期间CSTR中AUR和NUR分别为添加前的2.63和2.44倍,停止添加后又分别降为添加前的1.48和1.31倍.荧光原位杂交结果显示,添加期间,SBR中氨氧化菌(AOB)和亚硝酸盐氧化菌(NOB)的个数百分比分别为添加前的2.67和2.71倍,添加停止后,又分别降至原来的2.14和1.95倍;CSTR中AOB和NOB分别为添加前的2.91和1.77倍,但在添加停止后CSTR中AOB和NOB分别降至添加前的1.25和1.50倍.因此,硝化菌添加期间,两种流态的添加效果接近,但是在添加停止后,由于流态对硝化菌中K-决策者(Nitrosospira、Nitrospira)和r-决策者(Nitrosomonas europaea、Nitrobacter)的选择作用,添加的硝化菌在完全混合式条件下比推流式条件下更容易被系统淘汰.
SBR and CSTR were operated under the same nitrogen load and added strength at 15 ℃ to compare the effects of two typical flow regimes (push flow and complete mixing) on the effect of nitrifying bacteria on enhanced nitrification. The results showed that SBR The ammonia oxidation rate (AUR) and nitrite oxidation rate (NUR) were 2.34 and 2.39 times before addition, respectively, and then decreased to 2.01 and 1.78 times before addition respectively. During the addition, AUR and NUR were 2.63 and 2.44 times before addition, and then dropped to 1.48 and 1.31 times before addition respectively.Fluorescence in situ hybridization results showed that during the addition period, AOB and NOB of SBR increased, Were 2.67 and 2.71 times before addition, respectively, after which they were reduced to 2.14 and 1.95 times, respectively. The AOB and NOB in CSTR were 2.91 and 1.77 times before addition respectively, but after the addition was stopped, CSTR The AOB and NOB decreased to 1.25 and 1.50 times before adding respectively.Therefore, the adding effect of the two kinds of fluid was similar during the addition of nitrobacteria, but after the stop of the addition, the nitrifying bacteria’s Nitrosospira , Nitrospira) and r-decision makers (Nitrosomonas europaea, Nit robacter), the added nitrifying bacteria are more easily eliminated by the system under fully mixed conditions than by push flow conditions.