目的 探讨过氧亚硝基阴离子（ＯＮＯＯ－）在气道上皮细胞损伤致气道高反应性中的作用。方法 以离体豚鼠气管条建立组织胺累积－ 浓度反应曲线，电镜观察气道上皮细胞的形态学变化。结果 豚鼠气管条与ＯＮＯＯ－ 孵育后，气管条的反应性增高，同时电镜观察有气道上皮细胞损伤脱落。结论ＯＮＯＯ－ 参与了气道上皮细胞的损伤，并导致了气道高反应性的形成。 Objective To investigate the role of peroxynitrite (ONOO-) in airway hyperresponsiveness induced by airway epithelial cell injury. Methods Histamine accumulation - concentration response curve was established with guinea pig tracheal strips. The morphological changes of airway epithelial cells were observed by electron microscope. Results After guinea pig tracheal strips were incubated with ONOO-, the reactivity of the tracheal strips increased. At the same time, the damage of airway epithelial cells was observed by electron microscopy. Conclusion ONOO- is involved in the injury of airway epithelial cells and leads to the formation of airway hyperresponsiveness.