论文部分内容阅读
1目的 研究 bcl- 2与 P5 3蛋白在血管免疫母细胞性淋巴结病 (AIL )中的表达 ,探讨 AIL的发生发展规律、病变性质及恶性转化的可能机制。 2方法 应用免疫组化染色、PCR技术检测 bcl- 2与 P5 3蛋白表达及bcl- 2 /Ig H融合基因 ,同时选择非霍奇金病 (NHL ) 2 0例及淋巴结反应性增生 10例作为对比研究。 3结果 AIL病人 bcl- 2与 P5 3蛋白阳性表达率分别为 6 3.6 % (14/2 2 ) ,31.8% (7/2 2 ) ,bcl- 2 ,P5 3蛋白表达在 AIL与 NHL间差异均有显著性 (P<0 .0 5 )。AIL中 bcl- 2 /Ig H融合基因检出率为 4.6 % (1/2 2 ) ,淋巴结反应性增生病人中也检出 bcl- 2 /Ig H融合基因 (1/10 )。 4结论 bcl- 2与 P5 3蛋白通过不同的机制影响细胞凋亡 ,均参与 AIL的发生发展及恶性转化 ,AIL可能为不同原因、不同性质的疾病共有的临床症候群。
Objective To study the expression of bcl-2 and P53 protein in vascular immunoblastic lymphadenopathy (AIL) and to explore the development and progression of AIL, the nature of lesions, and possible mechanisms of malignant transformation. 2 Methods Immunohistochemical staining and PCR were used to detect the expression of bcl-2 and P53 protein and bcl-2/IgH fusion gene. At the same time, 20 cases of non-Hodgkin’s disease (NHL) and 10 cases of reactive hyperplasia of lymph nodes were selected as Comparative Study. 3 Results The positive expression rate of bcl-2 and P53 protein in AIL patients were 6 3.6 % (14/2 2), 31.8% (7/2 2), and the difference between bcl-2 and P53 protein expression in AIL and NHL were both. Significant (P<0.05). The detection rate of bcl-2/IgH fusion gene in AIL was 4.6% (1/2 2), and bcl-2/IgH fusion gene (1/10) was detected in patients with lymph node reactive hyperplasia. 4 Conclusions bcl-2 and P53 protein affect cell apoptosis through different mechanisms and participate in the development and malignant transformation of AIL. AIL may be a clinical syndrome shared by different causes and diseases.