论文部分内容阅读
目的观察大鼠局灶性脑缺血损伤后神经功能、脑梗死体积、脂质过氧化的变化及复方当归注射液的干预作用。方法以线栓法构建SD大鼠大脑中动脉栓塞致局灶性脑缺血损伤模型,随机分为假手术组、模型组、复方当归注射液组(药物组)和阳性对照组。造模后1 h,假手术组和模型组予生理盐水腹腔注射,药物组和阳性对照组分别给予复方当归注射液和依达拉奉注射液腹腔注射,连续7 d,通过神经行为学评分判断大鼠神经功能变化,四氮唑红染色方法测定脑组织梗死体积,硫代巴比妥酸方法检测缺血侧脑组织中丙二醛(MDA)含量。结果脑缺血7 d后,药物组脑梗死体积比及神经功能指标均较模型组显著改善(P<0.05),MDA含量较模型组有一定下降趋势。结论复方当归注射液能显著改善局灶性脑缺血大鼠的神经行为学功能、减小脑梗死体积,并可能有减轻脂质过氧化的作用。
Objective To observe the changes of neurological function, cerebral infarction volume and lipid peroxidation after focal cerebral ischemia in rats and the effect of Fufang Danggui injection. Methods The model of focal cerebral ischemia induced by middle cerebral artery occlusion in rats was established by thread occlusion. The rats were randomly divided into sham-operated group, model group, Fufang Danggui injection group and positive control group. At 1 h after model establishment, sham-operation group and model group were given intraperitoneal injection of normal saline. The drug group and the positive control group were given intraperitoneal injection of Fufang Danggui Injection and Edaravone Injection, respectively, for 7 days. The neurobehavioral score The neurological changes of rats were observed. The infarct volume of brain tissue was measured by tetrazolium red staining and the content of malondialdehyde (MDA) in ischemic brain tissue was detected by thiobarbituric acid method. Results After cerebral ischemia for 7 days, the cerebral infarction volume ratio and neurological function indexes in the drug group were significantly improved compared with the model group (P <0.05). The content of MDA in the drug group showed a tendency of decreasing. Conclusion Fufang Danggui Injection can significantly improve neurobehavioral function, reduce the volume of cerebral infarction, and may reduce lipid peroxidation in focal cerebral ischemia rats.