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对已构建的同时表达乙型肝炎病毒ＳＳ１、麻疹病毒ＨＡ和Ｆ以及ＩＬ２的非复制型重组痘苗病毒ＶＨＦＩＬ２ΔＣＫＳＳ１，及其相应的复制型重组病毒ＶＨＦＩＬ２ＳＳ１，进行了兔皮内毒力、粘膜免疫和细胞免疫效果的比较研究。发现ＶＨＦＩＬ２ΔＣＫＳＳ１在兔皮内的毒力明显低于ＶＨＦＩＬ２ＳＳ１。ＶＨＦＩＬ２ΔＣＫＳＳ１经空肠内免疫家兔４周后，可在肠粘膜表面诱发特异性ＩｇＡ抗体，在血液中诱发特异性的ＩｇＧ抗体，其抗ＳＳ１和抗麻疹病毒ＩｇＡ和ＩｇＧ的抗体滴度与ＶＨＦＩＬ２ＳＳ１免疫组相同，抗痘苗病毒ＩｇＧ抗体比ＶＨＦＩＬ２ＳＳ１免疫组低１６倍。ＶＨＦＩＬ２ΔＣＫＳＳ１经腹腔注射免疫４周的小鼠脾细胞，可在体外刺激产生高水平的麻疹病毒及痘苗病毒特异性ＣＴＬ，与ＶＨＦＩＬ２ＳＳ１无显著性差异。这些结果初步证实了非复制型痘苗病毒载体作为多价疫苗载体的有效性及安全性。
On the constructed non-replicating recombinant vaccinia virus VHFIL2ΔCKSS1 and its corresponding replication-competent recombinant virus VHFIL2SS1, which simultaneously express hepatitis B virus SS1, measles virus HA and F and IL2, rabbits endotoxicity, mucosal immunity and cells Comparison of immune effects. The virulence of VHFIL2ΔCKSS1 in rabbit skin was found to be significantly lower than that of VHFIL2SS1. Immunization of rabbits with jejunal VHFIL2ΔCKSS1 for 4 weeks induced specific IgA antibodies on the intestinal mucosa surface and induced specific IgG antibodies in the blood. The antibody titers of anti-SS1 and anti-measles virus IgA and IgG were similar to those of VHFIL2SS1-immunized group Similarly, the anti-vaccinia virus IgG antibody was 16-fold lower than the VHFIL2SS1 immunization group. VHFIL2ΔCKSS1 immunized 4 weeks after intraperitoneal injection of mouse spleen cells can stimulate high-level measles virus and vaccinia virus-specific CTL in vitro stimulation, no significant difference with VHFIL2SS1. These results initially confirmed the effectiveness and safety of non-replicating vaccinia virus vectors as multivalent vaccine vectors.