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基因转录抑制剂α-amanitin和蛋白质合成抑制剂cycloheximide完全抑制了香石竹(Dianthuscaryophyllus L.cvs.White Sim and Sandrosa)花瓣对乙烯反应的症状,包括花瓣卷曲和细胞膜离子渗漏增加。观察到花中蛋白质合成能力随着花的衰老而降低,花对乙烯的敏感性随花的衰老而增加。但是用乙烯合成抑制剂aminooxyacetic acid(AOA)预处理切花,则改变了花对乙烯敏感性的变化趋势。常用的香石竹品种D.caryophyllus L.cv.White Sim花经AOA处理后,对乙烯的敏感性随着花的衰老而下降。这些结果揭示花对乙烯的敏感性可能受蛋白质合成能力影响。
The gene transcription inhibitor α-amanitin and the protein synthesis inhibitor cycloheximide completely inhibited the reaction of the petals of Dianthus caryophyllus L. cv. White Sim and Sandrosa to ethylene, including the petal curl and the increase of cell membrane ion leakage. It was observed that the ability of protein synthesis in flowers decreased with the senescence of flowers, and the sensitivity of flowers to ethylene increased with senescence of flowers. However, the pretreatment of cut flowers with the inhibitor of ethylene synthase (AOA) changed the trend of ethylene sensitivity. Common carnation varieties D.caryophyllus L.cv.White Sim flowers treated with AOA, the sensitivity of ethylene decreased with the senescence of flowers. These results reveal that flower-to-ethylene sensitivity may be affected by protein synthesis capacity.