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快原子轰击质谱法(FAB-MS)可用于生物液及组织匀浆提取物中内源性肽的定量测定,灵敏度约为ppb水平,若选择一特征的碎片离子,用稳定同位素标记类似物作内标,以MS-MS 法进行定量测定,则有极高的分子专一性。这一分析方法可用于脑组织、牙髓组织和脊髓液中内啡肽的测定。FAB-MS 法测定内源性肽的原理是用一束高动能原子轰击溶解在甘油基质中的样品,通过冲量传递使样品分子电离,产生正离子方式(-M+H)~+或负离子方式(M-H)~-。由FAB 产生的(M+H)~+或(M-H)~-在MS-MS 的第二个质谱计中得到其子离子谱。在倒
Fast atom bombardment mass spectrometry (FAB-MS) can be used for the quantitative determination of endogenous peptides in biological fluid and tissue homogenate extracts with sensitivity of about ppb level. If a characteristic fragment ion is selected, a stable isotope labeled analogue Internal standard, quantitative determination by MS-MS method, there is a very high molecular specificity. This method of analysis can be used for the determination of endorphins in brain tissue, pulp tissue and spinal fluid. The principle of FAB-MS for the determination of endogenous peptides involves bombarding a sample dissolved in a glycerol matrix with a high kinetic energy atom and ionizing the sample molecules by impulse transfer to produce a positive ion mode (-M + H) ~ + or negative ion mode (MH) ~ -. (M + H) ~ + or (M-H) ~ - produced by FAB gave its daughter ion spectrum in the second mass spectrometer of MS-MS. In the fall