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为揭示亚热带不同森林类型对土壤氨氧化菌群落特征的影响,采用荧光定量PCR以及PCR-DGGE技术研究了阔叶林、杉木林、马尾松林和毛竹林土壤氨氧化古菌和细菌丰度及古菌群落结构特征.结果表明:不同林分土壤中氨氧化古菌数量(1.62×106~1.88×107个·g-1干土)高于相应土壤中的氨氧化细菌(2.41×105~4.36×105个·g-1干土);毛竹林土壤氨氧化古菌数量显著高于杉木林,而后者又显著高于阔叶林和马尾松林,但氨氧化细菌数量在不同林分之间没有显著差异.DGGE图谱分析表明,不同林分土壤中氨氧化古菌的物种有所差异,且毛竹林和杉木林土壤古菌群落结构迥异.氨氧化古菌在亚热带主要林分土壤中表现出明显优势,且除植被类型外,土壤速效钾、pH和有机质是引起土壤氨氧化古菌群落结构及多样性变异的主要因素.
In order to reveal the effects of different forest types on the characteristics of soil ammonia oxidizing bacteria in the subtropical zone, the Ammonia-oxidizing archaea and bacteria abundance in the broadleaved forest, Chinese fir plantation, Pinus massoniana plantation and Phyllostachys pubescens plantation were studied by using fluorescence quantitative PCR and PCR-DGGE, The results showed that the number of Ammonia-oxidizing archaea (1.62 × 106-1.88 × g-1 g-1 dry soil) in different stand soils was higher than that of ammonia-oxidizing bacteria in the corresponding soil (2.41 × 105-4.46 × 105 · g-1 dry soil). The number of ammonia oxidizing archaea in the soil of bamboo forest was significantly higher than that of Chinese fir plantation, while that of the latter was significantly higher than that of the broad-leaved forest and the masson pine forest. However, the number of ammonia-oxidizing bacteria was not significant among different stands The results of DGGE analysis showed that there were differences in the archaeal ammonia species in different stand soils, and the archaeal community structure was very different in the Phyllostachys pubescens forest and Cunninghamia lanceolata forest. Ammonia oxidizing archaea showed obvious advantages in the main stand subtropical soils , And besides the types of vegetation, available potassium, pH and organic matter were the main factors that caused the variation of archaea ammonia-oxidizing community structure and diversity.