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利用Wx基因第1内含子第1位的G/T和第6外显子第1 132位的A/C碱基多态性,分别开发分子标记Wx-I和等位基因特异性分子标记Wx-a-F(Wx-b-F)/Wx-a-R,对76份水稻品种Wx基因多态性进行检测,并分析不同基因型与直链淀粉含量的关系。结果表明,供试品种中在Wx基因的这2个多态性位点上检测到了3种基因型(In1G-Ex6A、In1G-Ex6C和In1TEx6A),分别表现为高、中、低直链淀粉含量;籼稻中这3种基因型均存在,而供试爪哇稻和粳稻中分别只检测到In1G-Ex6C和In1T-Ex6A基因型;供试籼型杂交水稻亲本中,恢复系Wx基因以In1T为主,三系保持系均为In1G,而两系不育系同时含有这2种类型,说明两系不育系的Wx基因变异比三系不育系丰富。
The molecular markers Wx-I and allele-specific molecular markers were developed using G / T at position 1 of the first intron of Wx gene and A / C base polymorphism at the 132nd at exon 6 Wx-aF (Wx-bF) / Wx-aR was used to detect the polymorphism of Wx gene in 76 rice cultivars and the relationship between different genotypes and amylose content was analyzed. The results showed that three genotypes (In1G-Ex6A, In1G-Ex6C and In1TEx6A) were detected in the two polymorphic sites of Wx gene in the tested cultivars, which showed high, medium and low amylose content . All three genotypes were present in indica rice, but only In1G-Ex6C and In1T-Ex6A genotypes were detected in the tested Java and japonica rice varieties. For the indica hybrid rice parents, the restorer line Wx was mainly In1T , Three lines were maintained in line In1G, and two lines of male sterile lines contain both types, indicating two lines of male sterile lines Wx genetic variation than the three-line sterile line.