论文部分内容阅读
目的探讨肝素对ＨＳＣ生长、细胞外基质和基质金属蛋白酶基因表达的影响。方法用肝素和不用肝素对活化的ＨＳＣ进行处理。以直接细胞计数和ＢｒｄＵ标记免疫细胞化学染色检测细胞的生长情况，用免疫细胞化学染色和细胞原位核酸分子杂交分别检测Ⅰ、Ⅳ型前胶原蛋白、纤连蛋白和Ⅰ、Ⅳ型胶原、纤连蛋白、基质金属蛋白酶－２及膜型基质金属蛋白酶基因的表达，并用酶图法检测基质金属蛋白酶－２的活性。结果肝素使血清所致的ＨＳＣ生长速度减慢；肝素抑制ＨＳＣⅠ型前胶原、纤连蛋白的合成和Ⅰ型前胶原、纤连蛋白、膜型基质金属蛋白酶基因的表达，但对Ⅳ型胶原、基质金属蛋白酶－２ ｍＲＮＡ水平和基质金属蛋白酶－２活性无影响。结论肝素明显抑制血清所致的ＨＳＣ的生长；下调ＨＳＣ间质型胶原和ＦＮ的合成，并抑制膜型基质金属蛋白酶基因的表达。
Objective To investigate the effect of heparin on HSC growth, extracellular matrix and matrix metalloproteinase gene expression. Methods Activated HSCs were treated with heparin and without heparin. The cell growth was detected by direct cell counting and BrdU labeling immunocytochemistry. The expressions of type Ⅰ and Ⅳ procollagen, fibronectin, type Ⅰ and Ⅳ collagen, fibronectin and fibronectin were detected by immunocytochemistry and in situ hybridization The expression of connexin, MMP - 2 and MMP - 2 were detected by enzyme - linked immunosorbent assay (ELISA). Results Heparin decreased the serum HSC growth rate. Heparin inhibited the expression of HSC type Ⅰ procollagen and fibronectin, and the expression of procollagen type Ⅰ, fibronectin and membrane type matrix metalloproteinases. However, Matrix metalloproteinase-2 mRNA level and matrix metalloproteinase-2 activity had no effect. Conclusion Heparin can significantly inhibit the growth of serum-induced HSC, down-regulate the synthesis of interstitial collagen and FN, and inhibit the expression of membrane-type matrix metalloproteinase gene.