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该实验研究NLRP3基因多态性与中国汉族人群结肠癌之间的相关性。采用TaqMan检测法对中国汉族人群208例结肠癌患者和203名正常对照NLRP3的3个位点(rs4925648、rs10754558、rs10925019)进行关联分析,并使用SPSS软件进行单核苷酸多态性分析,比较病例组和对照组等位基因频率、基因型频率及单倍型的差异。结果发现,NLRP3上三个位点基因频率、基因型频率两组间差异不明显(P>0.05)。rs4925648与rs10925019的LD分析其D’值较大(D’=0.798)。但进一步对两位点的单倍型分析发现其各种组合均无统计学差异。该研究结果提示,NLRP3基因的三个位点与中国汉族人群结肠癌的发生相关性无统计学意义。
This study investigated the association between NLRP3 gene polymorphism and colon cancer in Chinese Han population. TaqMan test was used to analyze the association between 208 unrelated colon cancer patients and 203 NLRP3 normal controls (rs4925648, rs10754558, rs10925019) in Chinese Han population. SPSS software was used to analyze single nucleotide polymorphisms The frequencies of alleles, genotype frequencies and haplotype differences between case group and control group. The results showed that there was no significant difference between the two genotypes in NLRP3 (P> 0.05). The LD value of rs4925648 and rs10925019 is larger (D '= 0.798). However, further analysis of the haplotypes at the two loci showed no significant difference among the various combinations. The results suggest that NLRP3 gene of the three sites and Chinese Han population colon cancer incidence was not statistically significant.