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采用免疫组化ＡＢＣ－ＧＤＮ及图象分析技术研究家猫ＳＯＭ中耳粘膜ＳＰ含量的变化与中耳积液的关系，并观察ＳＰ受体拮抗剂Ｓｐａｎｔｉｄｅ及组胺Ｈ＿２受体阻滞剂Ｃｉｍｅｔｉｄｉｎ对中耳积液的影响。结果发现：在ＳＯＭ条件下，中耳粘膜ＳＰ含量有逐渐增多趋势，并和中耳积液量呈正相关（ｒ＝０．９７５４，ｐ＜０．０５）。每日腹腔注射１ｍｇ／ｍｌＳｐａｎｔｉｄｅ及１ｍｇ／ｍｌＣｉｍｅｔｉｄｉｎ均可使中耳职液明显减少，随着ＳＯＭ时间的延长，二者使中耳积液减少的量并不一致，Ｓｐａｎｔｉｄｅ减少中耳积液量更明显。结果提示：ＳＰ是ＳＯＭ的重要炎性介质，介导血管的通透性及腺体的分泌，ＳＰ通过组胺发挥其扩张血管增加血管通透性的作用。
The immunohistochemical ABC-GDN and image analysis techniques were used to study the relationship between SP content in middle ear mucosa and the middle ear effusion. Spantide and histamine H_2 receptor blocker Cimetidin Middle ear effusion. The results showed that the content of SP in the middle ear mucosa gradually increased under the SOM condition and was positively correlated with the volume of the middle ear effusion (r = 0.9754, p <0.05). Daily intraperitoneal injection of 1mg / mlSpantide and 1mg / mlCimetidin can significantly reduce the middle ear fluid, with the SOM time, both to reduce the amount of fluid in the middle ear is inconsistent, Spantide reduce the amount of fluid in the ear more obvious . The results suggest that: SP is an important inflammatory mediator of SOM, mediating the permeability of blood vessels and the secretion of glands. SP plays its role of expanding blood vessels to increase vascular permeability through histamine.