本文提出叶绿素a和b的二阶导数荧光分析法。本法以450nm为激发波长。叶绿素b在644nm(正峰)测定,叶绿素a不干扰,测定范围为1—300ppb。叶绿素a在676nm(负峰)测定,叶绿素b在该波长有一弱的正峰应予扣除,测定范围为4—400ppb。此法与分光光度法、同步荧光法同时对照试验,结果基本一致。 In this paper, the second derivative fluorescence analysis of chlorophyll a and b is proposed. The law to 450nm excitation wavelength. Chlorophyll a was measured at 644 nm (positive peak) and did not interfere with chlorophyll a in the range of 1-300 ppb. Chlorophyll a is measured at 676 nm (negative peak) and chlorophyll b has a weak positive peak at this wavelength which should be deducted with a determination range of 4-400 ppb. This method and spectrophotometry, simultaneous fluorescence control test at the same time, the results are basically the same.