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利用分子信标检测技术,结合建立的ING1分子信标／cRNA杂交标准曲线,定量检测了药物处理,基因转染和p53 RNA干扰(RNAi)等对肿瘤细胞ING1 mRNA表达水平的影响．结果发现:在低表达ING1的肿瘤细胞系MCF-7中,5-FU处理和ING1基因稳定转染均能诱导细胞中ING1 mRNA表达水平升高;在ING1表达水平正常的CNE2肿瘤细胞中,利用RNAi沉默p53表达引起ING1 mRNA表达水平降低．这些细胞中ING1 mRNA表达水平在7．7×10-16-53．4×10-16mol／μg总RNA．该方法不仅能从转录水平上为基因表达调控效果提供定量依据,而且有望用于信号通路途径中多基因转录水平的相互调节研究．
Using molecular beacon detection technique, combined with the established standard curve of ING1 molecular beacon / cRNA hybridization, the effects of drug treatment, gene transfection and p53 RNA interference (RNAi) on the expression of ING1 mRNA in tumor cells were quantitatively detected. The results showed that 5-FU treatment and stable ING1 gene transfection could induce the expression of ING1 mRNA in ING1-deficient tumor cell line MCF-7. In CNE2 tumor cells with normal expression of ING1, RNAi silencing p53 expression causes a decrease in ING1 mRNA expression. ING1 mRNA expression levels in these cells ranged from 7.7 × 10-16 to 53.4 × 10-16 mol / μg total RNA. This method not only provides a quantitative basis for the regulation of gene expression at the transcriptional level, but also is expected to be used to study the mutual regulation of multi-gene transcriptional levels in signaling pathways.