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同步荧光法可使荧光谱带窄化,因而可大大提高荧光分析法的选择性(见图1).通常在进行同步扫描时所采用的发射与激发波长差△λ值较小(一般为0—5nm),导致溶剂散射光(主要是瑞利散射)对同步荧光信号的严重干扰,从而大大损害了分析灵敏度与检测限.广义地说,溶剂的散射光可分为两部分:一是散射光的总水平;另一是散射光的波动.二者抑制其一均可提高方法的检测灵敏度.我们曾报道,根据散射光与荧光的偏振性质的差异采用偏振
The synchronous fluorescence method can narrow the fluorescence spectrum, which can greatly improve the selectivity of fluorescence analysis (see Figure 1). Usually used in synchronous scanning when the emission and excitation wavelength difference △ λ value is small (usually 0 -5nm), resulting in solvent-scattered light (mainly Rayleigh scattering) on the synchronous fluorescence signal of the serious interference, which greatly undermined the analytical sensitivity and detection limits. Broadly speaking, the solvent scattering light can be divided into two parts: First, the scattering The general level of light and the other is the fluctuation of the scattered light, both of which inhibit the detection sensitivity of the method.We have reported that depending on the difference of the polarization properties of the scattered light and the fluorescence, the polarization