论文部分内容阅读
目的探析荧光定量PCR(Fluorescence quantitative PCR,FQ-PCR)检测乙肝病毒(hepatitis B virus,HBV)的方法,并分析其临床应用价值。方法选取385例体检显示乙肝表面抗原阳性患者血清标本,使用FQ-PCR及ELISA法对HBV进行检测,探讨FQ-PCR的检测方法、阳性率及临床意义。结果 FQ-PCR检测382例血清标本共检出231例阳性标本,阳性率60.5%;FQ-PCR对HBsAg(+),HBeAg(+),HBcAb(+)及HBsAg(+),HBeAg(+)检测阳性率较高,HBsAg、HBeAg、HBcAb均为阳性(大三阳)组阳性检出率最高,且各组与大三阳组HPV-DNA拷贝数存在显著统计学差异(P<0.05),HBsAb阳性组HPV-DNA阳性检出率较低。结论 FQ-PCR能够直观地反映HBV在肝细胞内的复制状态和传染性,指导临床用药,其诊断及指导意义均高于ELISA法,且操作方便、检测速度快,值得临床广泛推广。
Objective To investigate the detection of hepatitis B virus (HBV) by Fluorescence quantitative PCR (FQ-PCR) and to analyze its clinical value. Methods 385 cases of hepatitis B surface antigen positive patients were selected for the serum samples. FQ-PCR and ELISA were used to detect HBV. The detection methods, positive rate and clinical significance of FQ-PCR were explored. Results FQ-PCR was used to detect the positive rate of HBsAg (+), HBeAg (+), HBcAb (+) and HBsAg (+), HBeAg The detection rate was high, the positive rate of HBsAg, HBeAg and HBcAb was the highest, and the HPV-DNA copy number of each group had significant difference (P <0.05) HPV-DNA positive rate was lower in HBsAb positive group. Conclusions FQ-PCR can directly reflect the replication state and infectivity of HBV in hepatocytes and guide the clinical medication. The diagnostic and instructive significance of FQ-PCR is higher than that of ELISA. It is easy to operate and has high detection speed. It is worthy of clinical promotion.