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Objective Aquaporin-4(AQP4),the main water channel protein in the brain,plays a critical role in water homeostasis and brain edema.Here,we investigated its role in the inflammatory responses after focal cerebral ischemia. Methods In AQP4-knockout(KO) and wild-type mice,focal cerebral ischemia was induced by 30 min of middle cerebral arterial occlusion(MCAO).Ischemic neuronal injury and cellular inflammatory responses,as well as the expression and localization of cysteinyl leukotriene CysLT_2 and CysLT_1 receptors,were determined at 24 and 72 h after MCAO. Results AQP4-KO mice showed more neuronal loss,more severe microglial activation and neutrophil infiltration,but less astrocyte proliferation in the brain after MCAO than wild-type mice.In addition,the protein levels of both CysLT_1, and CysLT_2 receptors were up-regulated in the ischemic brain,and the up-regulation was more pronounced in AQP4-KO mice.The CysLT_1,and CysLT_2 receptors were primarily localized in neurons,microglia and neutrophils;those localized in microglia and neutrophils were enhanced in AQP4-KO mice.Conclusion AQP4 may play an inhibitory role in postischemic inflammation.
Objective Aquaporin-4 (AQP4), the main water channel protein in the brain, plays a critical role in water homeostasis and brain edema. Here, we investigated its role in the inflammatory responses after focal cerebral ischemia. Methods In AQP4-knockout (KO ) and wild-type mice, focal cerebral ischemia was induced by 30 min of middle cerebral arterial occlusion (MCAO). Ischemic neuronal injury and cellular inflammatory responses, as well as the expression and localization of cysteinyl leukotriene CysLT_2 and CysLT_1 receptors, were determined at 24 and 72 h after MCAO. Results AQP4-KO mice showed more neuronal loss, more severe microglial activation and neutrophil infiltration, but less astrocyte proliferation in the brain after MCAO than wild-type mice. In addition, the protein levels of both CysLT_1, and CysLT_2 receptors were up-regulated in the ischemic brain, and the up-regulation was more pronounced in AQP4-KO mice. CysLT_1, and CysLT_2 receptors were primarily localized in neurons, microglia and neutrophils; those localized in microglia and neutrophils were enhanced in AQP4-KO mice. Confocal AQP4 may play an inhibitory role in postischemic inflammation.