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目的:探讨海狗油软胶囊对内毒素的主要成份脂多糖(lipopolysaccharide,LPS)诱导大鼠形成急性肺损伤(acute lung injury,ALI)的保护作用及其作用机制。方法:SD大鼠分为6组:空白对照组、模型对照组、阳性对照组、海狗油高剂量组(H)、中剂量组(M)和低剂量组(L),除空白对照组外,其余大鼠尾静脉注射(iv)LPS 6 mg·kg-1造成急性肺损伤模型,造模前1周,分别灌胃(ig)海狗油1,0.5和0.25 g·kg-1,qd,连续7 d,末次给药1 h后LPS造模,阳性对照组造模同时ivω-3鱼油脂肪乳注射液1 g·kg-1,造模24 h后大鼠眼静脉丛取血并用二氧化碳窒息处死,测定各组大鼠血清肿瘤坏死因子(tumor necrosis factor,TNF-α)、白细胞介素1(interleukin-1,IL-1)和白细胞介素6(interleukin-6,IL-6),测肺脏湿重和干重比值(W/D),观察各组大鼠肺组织的病理改变等。结果:造模后大鼠体温升高,海狗油各剂量组与模型组相比,体温显著降低(P<0.01);核转录调节因子(NF-κB)和致炎细胞因子TNF-α,IL-1和IL-6都有不同程度地表达下调;W/D值显著降低(P<0.05)。各组大部分肺泡结构尚好,肺组织损伤程度低,明显好于模型组。结论:海狗油软胶囊对大鼠急性肺损伤具有保护作用,其作用机制可能与抑制NF-κB信号通路、抑制炎症细胞因子的表达有关。
Objective: To investigate the protective effect and protective mechanism of seal oil capsules on rats with acute lung injury (ALI) induced by lipopolysaccharide (LPS), a major component of endotoxin. Methods: The SD rats were divided into 6 groups: control group, model control group, positive control group, high oil seal oil group (H), medium dose group (M) and low dose group (Iv) LPS 6 mg · kg-1 was injected into tail vein of rats to induce acute lung injury. One week before modeling, After 7 days of continuous administration, LPS was made at 1 h after the last administration, and at the same time the positive control group was given iv g-1 fish oil emulsion injection 1 g · kg-1. After 24 h, ophthalmic plexus blood was drawn from rats and asphyxiated with carbon dioxide The rats were sacrificed and the serum levels of tumor necrosis factor (TNF), interleukin-1 (IL-1) and interleukin-6 (IL-6) Lung wet weight and dry weight ratio (W / D), observe the pathological changes of lung tissue in each group. Results: The body temperature of rats in model group was significantly lower than that in model group (P <0.01). The nuclear transcription factor (NF-κB) and pro-inflammatory cytokines TNF-α, IL -1 and IL-6 were down-regulated to some extent; the W / D value was significantly decreased (P <0.05). Most of the alveolar structure in each group is still good, low degree of lung tissue damage was significantly better than the model group. Conclusion: The fur seal oil soft capsule has protective effect on acute lung injury in rats. Its mechanism may be related to the inhibition of NF-κB signaling pathway and the inhibition of the expression of inflammatory cytokines.