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[目的]通过蛋白质组学方法探讨Her-2阳性与Her-2阴性人乳腺癌组织蛋白质组的差异表达。[方法]采用顺序抽提的方法将细胞总蛋白分级为水溶性和疏水性两个组分,并应用双向电泳方法对其进行分离,以获取蛋白质的表达谱;通过软件分析,找出表达量存在显著差异的蛋白质点;再应用基质辅助激光解吸电离飞行时间质谱与生物信息学相结合的方法对差异表达蛋白点进行分析鉴定。[结果]双向电泳图谱分析表明对水溶性蛋白质和疏水性蛋白都取得了很好的分离,并成功鉴定出7个差异表达蛋白,其中4个下调,3个上调。[结论]乳腺癌的发生发展与蛋白的表达改变有关,这些蛋白质参与信号传导、浸润转移及能量代谢等多个方面。
[Objective] To explore the proteomic differences between Her-2-positive and Her-2-negative human breast cancer tissues by proteomics. [Method] The total cellular protein was fractionated into two components, water-soluble and hydrophobic, using sequential extraction method and separated by two-dimensional electrophoresis to obtain the protein expression profile. Through software analysis, the expression level There are significant differences in protein spots; and then using matrix-assisted laser desorption / ionization time-of-flight mass spectrometry combined with bioinformatics methods to differentially expressed protein spots were identified. [Result] The results of two - dimensional gel electrophoresis showed that both water - soluble protein and hydrophobic protein were well separated and seven differentially expressed proteins were successfully identified, of which four were down - regulated and three were up - regulated. [Conclusion] The occurrence and development of breast cancer are related to the changes of protein expression. These proteins are involved in many aspects such as signal transduction, invasion and metastasis and energy metabolism.