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用于致突性研究的第一个哺乳动物细胞系是中国地鼠肺成纤维细胞(V_(79))。Chu等首先定量研究了化学物诱发V_(79)细胞的突变,发现表达时间、细胞密度、选择剂浓度等是影响实验的重要因素。目前使用的方法已有了较大的改进,本文仅就V_(79)细胞致突变试验的方法学作一简要综述。
The first mammalian cell line used for the mutagenicity study was Chinese hamster lung fibroblasts (V_ (79)). Chu et al first quantified the chemical-induced mutation of V_ (79) cells and found that the expression time, cell density, concentration of selective agent and other factors are important factors in the experiment. The current methods have been greatly improved, this article only V_ (79) cell mutagenicity method for a brief review.