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目的 观察和比较兔眼视网膜各层组织对高眼压的易损性。方法 对 16只成年灰兔实验眼前房内注射 2 %甲基纤维素 0 10～ 0 15ml,制作成高眼压模型。分期制作眼球标本 ,常规石蜡切片、HE染色。采用计算机图像分析技术对兔视网膜各组织参数进行定量分析。结果 兔视网膜各组织呈现程度不等的萎缩。以视网膜神经节细胞 (retinalganglioncells ,RGCs)密度和视网膜神经纤维层 (retinalnervefiberlayer,RNFL)厚度改变最为明显 ,减少率分别为 74 3%和 6 1 9%。其他依次为 :视网膜内层 (4 2 4% )、神经上皮全层 (35 0 % ) ,视网膜外层改变最不明显 (2 4 2 % ) ;实验眼RNFL的厚度改变与RGCs的密度变化具有显著相关性 (r=0 6 8,P <0 0 1) ;RNFL厚度变化与眼压高低程度密切相关 (F =8 97,P <0 0 1)。结论 视网膜各层组织在高眼压状态下呈现不同的易损性 ;RNFL厚度改变是高眼压损害视网膜的敏感指标之一。
Objective To observe and compare the vulnerability of rabbit retina to ocular hypertension. Methods Sixteen adult gray rabbit rabbits were injected intraperitoneally with 2% methylcellulose 0 10 ~ 0 15ml and made into intraocular pressure model. Phased production of eye specimens, conventional paraffin sections, HE staining. Using computer image analysis technique to quantitatively analyze the parameters of rabbit retina. Results Rabbit retina show varying degrees of tissue atrophy. The most obvious changes were the density of retinal ganglion cells (RGCs) and the thickness of retinal nerve fiber layer (RNFL), with a reduction rate of 74.3% and 61.9% respectively. The other followed were: the inner retina (42.4%), the full thickness of the neuroepithelium (35%), and the least change of the outer retina (24.2%). The thickness of RNFL and the density of RGCs (R = 0.68, P <0.01). The thickness of RNFL was closely related to the level of intraocular pressure (F = 897, P <0.01). Conclusion All layers of retina show different vulnerability under high intraocular pressure. The change of thickness of RNFL is one of the sensitive indicators of intraocular pressure damage to the retina.