【摘 要】
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Background:Fusion genes may play an important role in tumorigenesis,prognosis,and drug resistance;however,studies on fusion genes in endometrial cancer (EC) are
【机 构】
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Department of Obstetrics and Gynecology, Peking University People’s Hospital, Beijing 100044, China
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Background:Fusion genes may play an important role in tumorigenesis,prognosis,and drug resistance;however,studies on fusion genes in endometrial cancer (EC) are rare.This study aimed to identify new fusion genes and to explore their clinical significance in EC.Methods:A total of 28 patients diagnosed with EC were enrolled in this study.RNA sequencing was used to obtain entire genomes and transcriptomes.STAR-comparison and STAR-fusion prediction were applied to predict the fusion genes.Chi-square tests and Student t tests were used to verify the clinical significance with SPSS 13.0 software.Results:New fusion genes were found,and the number of fusion genes varied from 3 to 110 among all patients with EC.The type of fusion genes varied and included messenger RNA (mRNA)-mRNA,long non-coding RNA (lncRNA)-lncRNA,and lncRNA-mRNA.There were six fusion genes with high fusion rates,namely,RP11-123O10.4-GRIP1,RP11-444D3.1-SOX5,RP11-680G10.1-GSE1,NRIP1-AF127936.7,RP11-96H19.1-RP11-446N19.1,and DPH7-PTP4A3.Further studies showed that these fusion genes are related to stage,grade,and recurrence,in which NRIP1-AF127936.7 and DPH7-PTP4A3 were found only in stage Ⅲ patients with EC.DPH7-PTP4A3 was found in grades 2 and 3,and recurrent patients with EC.Conclusion:Fusion genes play an essential role in EC.Six genes that are overexpressed with high fusion rates are identified.NRIP1-AF127936.7 and DPH7-PTP4A3 might be related to stage,and DPH7-PTP4A3 be related to grade and recurrence.
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