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小鼠的采血方法不少,但所得血液由於血流不畅或与皮毛及组织接触,导致组织因子混入及凝血Ⅻ因子激活,使小鼠凝血象测定不易准确。我们改进了采血及部分测定方法,用颈动脉塑料插管放血,采血量达到2-2.5ml,一次采血可进行十二个项目的凝血测定,现将C_3H.BALB/c.BG1.YG1.G15.SW1六种不同近交品系小鼠的测定结果及方法报导如下,提供有关方面参考。
There are many ways to collect blood from mice, but the resulting blood has poor blood flow or contact with the skin and tissues, resulting in the incorporation of tissue factor and the activation of coagulation factor XII, making it difficult to accurately measure blood clotting in mice. We improved blood collection and partial measurement methods. Blood was taken from a plastic cannula through a carotid artery and the blood volume was 2-2.5 ml. A blood sample can be assayed for coagulation in twelve items. Now C_3H.BALB/c.BG1.YG1.G15 The results and methods of determination of mice in six different strains of inbred strains of SW1 are reported below and provide relevant references.