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利用酶联免疫吸附测定法 (ELISA)结合卡那霉素涂棉花的方法 ,检测转基因抗虫棉组织中Bt杀虫蛋白的表达。这一检测方法不仅可以快速、方便地对转基因抗虫棉组织中Bt杀虫蛋白表达量进行定性、定量测定 ,也对转基因抗虫棉的选育和抗虫性测定提供了依据。
The expression of Bt insecticidal protein in transgenic Bt cotton was detected by enzyme-linked immunosorbent assay (ELISA) combined with kanamycin-coated cotton. This method not only can be used for the qualitative and quantitative determination of Bt insecticidal protein in transgenic Bt cotton quickly and conveniently, but also provide a basis for the selection and insect resistance determination of Bt cotton.