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目的 :研究重组人骨形成蛋白 2 (rhBMP2 )诱导小鼠颅神经嵴细胞 (CNCC)分化后所产生的神经元类型是否为肾上腺能神经元。方法 :用 5 0ng/mlrhBMP2 诱导CNCC分化为神经元。提取诱导前后细胞内的总RNA ,用反转录聚合酶链反应 (RT -PCR)分别检测细胞中酪氨酸羟化酶 (TH )和γ -氨基丁酸受体 (GABAAR )亚单位的表达情况。结果 :诱导前的CNCC中 ,TH和GABAAR亚单位均无mRNA水平的表达 ,诱导后二者均有表达 ,但表达的量均低于脑组织中的表达。结论 :rhBMP2 诱导CNCC后所分化的神经元为肾上腺能神经元 ,但在功能上可能弱于正常的肾上腺能神经元。
Objective: To investigate whether the type of neurons produced by rhBMP2-induced cranial neural crest cells (CNCC) differentiate into adrenoceptors. Methods: CNCCs were induced to differentiate into neurons with 50 ng / ml rhBMP2. Total RNA was extracted from the cells before and after induction, and the expression of tyrosine hydroxylase (TH) and gamma-aminobutyric acid receptor (GABAAR) subunits were detected by reverse transcriptase polymerase chain reaction (RT-PCR) Happening. Results: There was no expression of TH and GABAAR subunits in the pre-induction CNCC. After induction, both of them were expressed, but the expression levels were lower than those in brain tissue. CONCLUSION: The neurons differentiated from rhBMP2 induced by CNCC are adrenal neurons, but they may be weaker than the normal adrenoceptors in function.