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为应用猕猴和树鼩动物模型研究毒品成瘾对神经/免疫系统的影响提供基础数据,对大麻素及阿片受体在正常猕猴和树鼩神经系统和免疫系统的表达进行初步确定。采集正常猕猴和树鼩新鲜组织(皮质、小脑、脑干、海马、脊髓、脾脏),应用半定量逆转录PCR和实时定量PCR的方法检测大麻素与阿片受体mRNA在猕猴和树鼩各组织中的表达情况。猕猴脑部各区包括脾脏均表达大麻素受体1(CNR1),而大麻素受体2(CNR2)只表达于脾脏内。三类阿片受体中,mu(μ)受体表达最为广泛,在以上各组织中均有表达;delta(δ)受体表达的组织最少,只在海马表达;kappa(κ)受体表达介于两者之间,分别在皮质、小脑、脑干、脊髓中表达。在树鼩组织中,CNR1和CNR2表达于整个大脑重要脑区中,且CNR1表达量高于同一区域内CNR2表达的量;脾脏中CNR2的表达较高,而CNR1不表达。三类阿片受体只有检测到μ受体在脑部与脾脏表达,且在各个脑区的表达量明显高于脾脏的表达量;δ受体和κ受体在被检各个组织中均无表达。总体而言,两种大麻素受体在猕猴和树鼩体内表达情况与人类和鼠的情况类似,而三类阿片受体在猕猴体内表达情况与人类更为接近。猕猴和树鼩可能可用于人类毒品成瘾的研究;猕猴在某些神经受体的表达更接近人类,其在研究毒品成瘾的机理和对免疫系统的影响方面仍有不可替代的地位。
To provide basic data for studying the effects of drug addiction on the nervous system and immune system in macaque and shrew animal models and to initially determine the expression of cannabinoids and opioid receptors in the nervous system and immune system of normal macaque and tree shrew. The normal tissues of cynomolgus monkeys and tree shrews (cortex, cerebellum, brain stem, hippocampus, spinal cord and spleen) were collected and semi-quantitative RT-PCR and real-time quantitative PCR were used to detect the mRNA expression of cannabinoid and opioid receptor In the expression of the situation. Canine brain regions, including the spleen, express Cannabinoid Receptor 1 (CNR1), whereas Cannabinoid Receptor 2 (CNR2) is expressed only in the spleen. Among the three types of opioid receptors, the mu (μ) receptors were the most widely expressed and expressed in all the above tissues; the delta (δ) receptors expressed the least tissue and expressed only in the hippocampus; the kappa (κ) Between the two, respectively, in the cortex, cerebellum, brain stem, spinal cord expression. In tree shrew tissue, CNR1 and CNR2 were expressed in important brain regions of the whole brain, and CNR1 expression was higher than CNR2 expression in the same region; CNR2 expression was higher in spleen and CNR1 was not expressed. Only three kinds of opioid receptors were detected in the brain and spleen expression of μ receptors, and in all brain regions were significantly higher than the expression of spleen expression; δ receptor and κ receptor were not detected in all tissues tested . Overall, both cannabinoid receptors are expressed similarly in humans and mice in both rhesus and tree trunks, whereas the three opioid receptors are more closely expressed in humans than in humans. Rhesus macaques and tree shrews may be useful in the study of drug addiction in humans. Rhesus macaques are closer to humans in their expression of certain neural receptors and remain irreplaceable in the study of the mechanism of drug addiction and their impact on the immune system.