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为进一步了解骨骼肌组织的死后变化特点和规律及其与死后时间(PMI)的关系,本文选用成年实验大鼠。系列观察了死后0～96小时(0～96hpm)骨骼肌运动终板和肌纤维在不同温度条件下AChE(乙酰胆碱酯酶),SDH(琥珀酸脱氢酶),LDH(乳酸脱氢酶),Ca~(2+)-ATPase(钙激活三磷酸腺苷酶)的酶组织化学变化及运动终板突触素(synaptophysin,SYN)的免疫组织化学变化,并使用图像处理系统对上述酶反应结果进行了定量分析和比较。结果发现,上述改变与PMI有明显的相关关系,其中运动终板AChE因位于突触间隙的特殊位置以及自身的耐活性质,反应明确,稳定性高,降解缓慢显示更清晰和持久的降解曲线,可用作为推断死亡时间的标志酶。
In order to further understand the characteristics and rules of skeletal muscle after the death and its relationship with the postmortem interval (PMI), adult experimental rats were selected in this study. Series observed at 0 to 96 hours after the death (0 ~ 96hpm) skeletal muscle motor end plates and muscle fibers under different temperature conditions AChE (acetylcholinesterase), SDH (succinate dehydrogenase), LDH (lactate dehydrogenase) The enzymatic histochemical changes of Ca ~ (2 +) - ATPase and the synaptophysin synaptophysin (SYN) were detected by enzyme-linked immunosorbent assay (ELISA) Analysis and comparison. The results showed that there was a significant correlation between the above changes and PMI. The motor endocardium AChE was characterized by its specific location in the synaptic cleft as well as its own anti-live properties. The reaction was clear and stable, and the degradation showed a clearer and longer lasting degradation curve , Can be used as markers to infer the time of death enzyme.