论文部分内容阅读
构建了一对二英类化学物质敏感的人肝癌细胞株 ,用于二英类化学物质生物筛选和快速半定量检测 .首先构建一在二英增强子调控下的萤光素酶报告基因质粒 ,该质粒转染入人肝癌细胞株HepG2 ,筛选稳定转染细胞株 .2 ,3,7,8 四氯代二苯并二英 (TCDD)诱导稳定转染细胞株萤光素酶表达 ,发光检测萤光素酶活性 .该细胞株用于TCDD检测 ,检测下限为 1 1pmol L ,线性范围为 1～ 10 0pmol L .该细胞系的建立可用于二英类化学物质的生物筛选和快速半定量检测
A pair of human liver cancer cell strains that are sensitive to chemical substances belonging to the dioxin category was constructed for biological screening and rapid semi-quantitative detection of Dioxin-type chemical substances. First, a luciferase assay under the control of the dioxin enhancer was constructed. Gene plasmid, which was transfected into human hepatocellular carcinoma cell line HepG2, screened for stable transfected cell lines. 2, 3, 7, 8 tetrachlorodibenzo-dioxime (TCDD) induced stably transfected cell line luciferase Expression, luminescence detection of luciferase activity. The cell line was used for TCDD detection with a detection limit of 11 pmol L and a linear range of 1 to 100 pmol L. This cell line was established for the biological screening of Dioxin-type chemicals. And rapid semi-quantitative detection