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目的研究青蒿琥酯(Art)及其联合顺铂(DDP)对人肝癌Hep G2细胞增殖和凋亡的影响。方法分别采用不同浓度的Art(0.16、0.8、4、20、100μmol/L)、DDP(0.16、0.8、4、20、100 mg/L)及Art(4μmol/L)和DDP(4 mg/L)联合处理Hep G2细胞24 h、48 h和72 h,MTT实验检测细胞增殖情况,流式细胞术检测Art(4μmol/L)组、DDP(4mg/L)组和Art(4μmol/L)+DDP(4 mg/L)联合处理组细胞48 h的凋亡状况。结果随着处理时间延长(24~72h),Art和DDP对Hep G2细胞生长的抑制/杀伤作用明显增强;它们在暴露72 h的条件下分别在0.16μmol/L和0.8 mg/L以上浓度具有浓度依赖性细胞毒作用。Art联合DDP处理细胞组对人肝癌Hep G2细胞的增殖抑制作用比单用Art或DDP组更强(P<0.05);人肝癌Hep G2细胞48 h的早期凋亡率分别为:Art组23.5%,DDP组27.8%,Art+DDP联合用药组49.7%,差异有统计学意义(P<0.05)。结论青蒿琥酯可抑制人肝癌Hep G2细胞增殖并诱导其凋亡;它与顺铂联合应用可能增强后者对人肝癌Hep G2细胞的细胞毒作用。
Objective To study the effects of Artesunate (Art) and its combination with cisplatin (DDP) on the proliferation and apoptosis of Hep G2 cells. Methods Different concentrations of Art (0.16,0.8,4,20,100μmol / L), DDP (0.16,0.8,4,20,100 mg / L) and Art (4μmol / L) and DDP ) Treatment of Hep G2 cells for 24 h, 48 h and 72 h, MTT assay was used to detect the proliferation of Hep G2 cells. Flow cytometry was used to detect the effects of Art (4μmol / L), DDP (4mg / L) DDP (4 mg / L) combined treatment group of 48 h apoptosis. Results With the prolongation of treatment time (24 ~ 72h), Art and DDP significantly inhibited the growth and proliferation of Hep G2 cells. After exposure for 72 h, Art and DDP had the effects of 0.16μmol / L and 0.8 mg / L respectively Concentration-dependent cytotoxicity. Art combined with DDP treated cells inhibited the proliferation of human hepatocellular carcinoma Hep G2 cells more than those treated with Art or DDP alone (P <0.05). The early apoptosis rates of Hep G2 cells treated with Art combined with DDP were 23.5% 27.8% in DDP group and 49.7% in Art + DDP combined group. The difference was statistically significant (P <0.05). Conclusion Artesunate can inhibit Hep G2 cell proliferation and induce apoptosis of Hep G2 cells, and its combination with cisplatin may enhance the cytotoxicity of the latter on human hepatoma Hep G2 cells.