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目的:研究血液透析对36例终末期肾病患者血中高级糖基化终产物的清除效果。方法:分别用荧光分光光谱、流动注射分析法和酶联免疫吸附法测定终末期肾病患者血中高级糖基化终产物的水平及比较血液透析前后的变化。结果:荧光分光光谱、流动注射法和酶联免疫吸附法测定的终末期肾病患者血中高级糖基化终产物的水平显著高于对照组,分别为(25±5)vs(7.5±1.5),(6.1±1.8)vs(1.4±0.5)和(37±20)vs(9±10)kU/L,透析后上述水平分别降到(22±6),(4.2±1.4)和(19±14)kU/L。结论:血液透析能有效地清除终末期肾病患者血中增加的高级糖基化终产物。
Objective: To study the effect of hemodialysis on the clearance of advanced glycation end products in 36 patients with end-stage renal disease. Methods: The levels of advanced glycosylation end products (AGEs) in blood of patients with end-stage renal disease were measured by fluorescence spectroscopy, flow injection analysis and enzyme-linked immunosorbent assay (ELISA) respectively, and the changes before and after hemodialysis were compared. Results: The levels of advanced glycosylation end products in patients with end-stage renal disease were significantly higher than those in the control group (25 ± 5 vs 7.5 ± 1.5, respectively) by fluorescence spectroscopy, flow injection and enzyme-linked immunosorbent assay , (6.1 ± 1.8) vs (1.4 ± 0.5) and (37 ± 20) vs (9 ± 10) kU / L after dialysis. The levels were decreased to (22 ± 6), (4.2 ± 1.4) and 14) kU / L. Conclusion: Hemodialysis can effectively remove the advanced glycosylation end products in the blood of patients with end stage renal disease.