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目的:研究微小RNA-21(miR-21)在妊娠期肝内胆汁淤积症(ICP)患者中的表达及其临床检测的意义。方法:选取2012年2月至2014年11月在中山市博爱医院诊断为ICP患者的外周血58例及同期住院的58例正常产妇,提取58例妊娠期肝内胆汁淤积症患者及58例同期正常妊娠妇女外周血总RNA,q RT-PCR检测miR-21的表达,统计分析miR-21表达与ICP患者临床参数的关系及与甘胆酸、总胆汁酸水平的关系。结果:miR-21在妊娠期肝内胆汁淤积症患者中明显高于正常妊娠妇女。血清miR-21水平与妊娠期肝内胆汁淤积症患者的年龄、孕周、经产与否无关,但是与围生儿预后呈负相关。ICP患者血清miR-21高表达则患者甘胆酸及总胆汁酸水平较对照组高。结论:血清miR-21在ICP患者中含量增加,与围生儿预后呈负相关。检测血清miR-21在ICP患者围生儿预后预测及与甘胆酸及总胆汁酸联合在ICP患者的诊断中发挥重要作用。
Objective: To investigate the expression of microRNA-21 (miR-21) in patients with intrahepatic cholestasis of pregnancy (ICP) and its clinical significance. Methods: From February 2012 to November 2014, 58 cases of peripheral blood from ICP patients and 58 normal women were enrolled in Pok Oi Hospital of Zhongshan City. 58 pregnant women with intrahepatic cholestasis of pregnancy and 58 normal pregnant women The expression of miR-21 was detected by q RT-PCR in normal pregnant women’s peripheral blood. The relationship between the expression of miR-21 and the clinical parameters of ICP patients and the relationship with miR-21 and total bile acid were analyzed statistically. Results: miR-21 was significantly higher in patients with intrahepatic cholestasis of pregnancy than in normal pregnant women. Serum miR-21 levels in patients with intrahepatic cholestasis of pregnancy age, gestational age, after birth or not, but with a negative correlation with the prognosis of perinatal children. Patients with ICP patients with high expression of miR-21 in patients with glyceric acid and total bile acid levels higher than the control group. Conclusion: The serum level of miR-21 is increased in patients with ICP, which is negatively correlated with the prognosis of infants. Detection of Serum miR-21 Predicts Perinatal Prognosis in Patients with ICP and Combined with Glycocholic Acid and Total Bile Acid Play an Important Role in the Diagnosis of ICP Patients.