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采用环境控制生长室控制CO2浓度的方法,研究了CO2浓度(350~400μmolmol-1和680~750μmolmol-1对植物根内丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)群落的影响。12种宿主植物于CO2浓度不同的生长室栽培180d后收获取样,通过CTAB法提取共生菌根内丛枝菌根真菌的DNA,由特异引物U1/U2扩增编码核糖体28S大亚基的rDNA部分序列,并进行DGGE电泳分析。结果表明,12种植物根内的AMF存在特异的AMF类群(unique species group,US)和共有类群(commons pecies group,CS),而且CO2浓度倍增使US减少而CS增加。与350μmolmol-1对照相比,700μmolmol-1处理的玉米、刺苋、大豆、陆稻、无芒稗、黑麦草6种植物的AMF群落多样性下降,下降幅度分别达27·12%、16·84%、10·12%、8·62%、8·58%和2·67%;白车轴、牛筋草、早熟禾、鼠曲草、野燕麦、北美车前6种植物的AMF群落多样性上升,分别达76·26%、28·50%、17·60%、15·08%、1·46%和0·96%。CO2倍增处理后12种植物的AMF多样性平均指数略呈上升趋势。研究指出未来环境变化(如CO2增加)将影响AMF群落结构从而影响菌根共生体的形成。
The effects of CO2 concentration (350 ~ 400μmolmol-1 and 680 ~ 750μmolmol-1) on the arbuscular mycorrhizal fungi (AMF) community in plant roots were studied by using the method of CO2 concentration control in the growth chamber of the environment. The plants were harvested in a growth chamber of different CO2 concentrations for 180 days after harvesting. The DNA of the arbuscular mycorrhizal fungi in the symbiotic mycorrhizal fungi was extracted by CTAB method. The rDNA partial sequences encoding the 28S large subunit of ribosomes were amplified by specific primers U1 / U2. And DGGE analysis showed that there were specific AMF groups (US) and commons pecies group (AMF) in AMF of 12 plant species, and the doubling of CO2 concentration reduced US and increased CS. Compared with 350μmol mol-1 control, the AMF community diversity of 700 plants treated with 700μmol mol-1 maize, amaranth, soybean, upland rice, barnyardgrass and ryegrass decreased by 27 · 12% and 16 · 84%, 10 · 12%, 8.62%, 8.58% and 2.67%, respectively. The AMF community of the six species of white car axis, tendon grass, bluegrass, Rose by 76.26%, 28.5%, 17.60%, 15.08%, 1.46% and 0.96% respectively. The average AMF diversity index of 12 plants increased slightly after doubled treatment.The study indicated that the future environmental changes (such as CO2 increase) will affect AMF community structure and thus affect the formation of mycorrhizal symbionts.