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[目的]过度的新骨形成和骨吸收是强直性脊柱炎(AS)的重要病理特征。研究表明,AS病人滑膜的病理变化与该病的病变严重程度密切相关。本研究用蛋白质组学方法比较AS、类风湿性关节炎(RA)、骨关节炎(OA)滑膜组织的蛋白表达谱,筛选AS疾病相关的特异性表达蛋白。[方法]AS滑膜取自髋关节,RA和OA滑膜取自膝关节。提取每个滑膜(每种疾病n=10)的总蛋白,然后按等比例浓度混合分别制备每种疾病的样本池。用双向凝胶电泳分析AS、RA、OA滑膜的蛋白表达谱,用质谱分析方法鉴定AS中特异高表达的蛋白。用免疫组织化学法和蛋白免疫印迹法逐一分析靶蛋白在每个滑膜中的表。[结果]与RA和OA相比,蛋白质组学发现碳酸酐酶1(car-bonic anhydrase I,CA1)在AS滑膜中特异性高表达。免疫组织化学法和蛋白免疫印迹均证实CA1在AS滑膜中明显表达(P=0.01)。[结论]研究证实,CA1可以促进钙沉淀和骨吸收。AS关节滑膜组织中高表达CA1可能会通过刺激新骨形成和骨吸收而参与AS病理过程。
[Objective] Excessive new bone formation and bone resorption are important pathological features of ankylosing spondylitis (AS). Studies have shown that the pathological changes of synovium in patients with AS are closely related to the severity of the disease. In this study, proteomic profiling of AS, rheumatoid arthritis (RA) and osteoarthritis (OA) synovial tissue was compared by proteomics to screen for specific proteins associated with AS disease. [Methods] AS synovium was taken from the hip joint, RA and OA synovial membrane were taken from the knee joint. Total protein from each synovial membrane (n = 10 for each disease) was extracted and then pooled for each disease separately. The expression profiles of AS, RA and OA synovial membrane were analyzed by two-dimensional gel electrophoresis, and the proteins that were highly expressed in AS were identified by mass spectrometry. Immunohistochemistry and Western blotting were used to analyze the target proteins in each synovial membrane. [Results] Compared with RA and OA, proteomics showed that car-bonic anhydrase I (CA1) was highly expressed in AS synovium. Immunohistochemistry and Western blot confirmed that CA1 was significantly expressed in AS synovium (P = 0.01). [Conclusion] The study confirmed that CA1 can promote calcium precipitation and bone resorption. High expression of CA1 in synovial tissues of AS may be involved in the pathological process of AS by stimulating new bone formation and bone resorption.