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目的建立LC-MS/MS法同时测定人血浆中阿罗洛尔、阿替洛尔、比索洛尔和美托洛尔的浓度。方法人血浆样品用乙酸乙酯萃取后,选用ZORBAX SB C18(100 mm×2.1 mm,3.5μm)色谱柱,以甲醇-0.1%甲酸为流动相梯度洗脱,采用电喷雾离子化源、正离子方式和多反应监测(MRM)扫描方式进行监测。结果血浆中阿罗洛尔、阿替洛尔、比索洛尔和美托洛尔的线性范围均为1.0~500μg·L-1(r>0.99),定量下限均为1.0μg·L-1;平均回收率94.4%~107.9%;日内、日间RSD均<15%;提取回收率在78.8%~89.4%。结论该方法快速、灵敏、准确,专属性强,重复性好,适用于人血浆中同时测定阿罗洛尔、阿替洛尔、比索洛尔和美托洛尔浓度。
Objective To establish a simultaneous LC-MS / MS method for the determination of arotinolol, atenolol, bisoprolol and metoprolol in human plasma. Methods Human plasma samples were extracted with ethyl acetate. The mobile phase consisted of ZORBAX SB C18 (100 mm × 2.1 mm, 3.5 μm) and methanol-0.1% formic acid as eluent. The electrospray ionization Mode and multi-reaction monitoring (MRM) scan mode. Results The linear ranges of arotinolol, atenolol, bisoprolol and metoprolol in plasma ranged from 1.0 to 500 μg · L -1 (r> 0.99), and the lower limits of quantitation were 1.0 μg · L -1 respectively. The average The recoveries ranged from 94.4% to 107.9%. The daily and intraday RSDs were all less than 15%. The recovery rates ranged from 78.8% to 89.4%. Conclusion The method is rapid, sensitive, accurate, specific and reproducible. It is suitable for the simultaneous determination of arotinolol, atenolol, bisoprolol and metoprolol in human plasma.