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目的了解ＴＴＶ感染状况，探讨ＴＴＶ致病性及对干扰素治疗的应答。方法采用ＰＣＲ和原位杂交方法检测血清和肝组织中ＴＴＶ ＤＮＡ，回顾分析８例ＴＴＶ／ＨＣＶ感染者干扰素治疗后病毒及ＡＬＴ应答状况。结果各型肝病患者（３６．７％～４２．８％）和职业供血员（４３．２％）ＴＴＶ感染率均显著高于义务供血员（健康对照人群，５．６％）。部分感染者采用斑点杂交方法可自血清中检出ＴＴＶ ＤＮＡ（３／３０，１０．０％），提示存在较高的血清病毒负荷， １６例慢性非甲～戊型肝炎患者及１６例肝炎后肝硬化患者肝组织以原位杂交检测，各６例ＴＴＶ ＤＮＡ阳性（３７．５％）。 ８例ＴＴＶ／ＨＣＶ混合感染者经２４周疗程干扰素治疗后，６例维持持久ＡＬＴ应答（停药后２４周ＡＬＴ正常），治疗１２周时， ８例血清ＴＴＶ ＤＮＡ均阴转，但 ２例停药后复阳， ＡＬＴ无应答的２例患者， ＨＣＶ ＲＮＡ呈阳性但ＴＴＶ ＤＮＡ阴怀；而ＴＴＶ ＤＮＡ阳转的２例患者ＡＬＴ均维持应答。结论 ＴＴＶ感染在肝病患者和职业供血员中较普遍， ＴＴＶ可在肝组织中活跃复制，其对干扰素治疗反应较敏感，ＴＴＶ／ＨＣＶ重叠感染时ＴＴＶ无明显致肝损伤作用。
Objective To understand the status of TTV infection and to explore the pathogenicity of TTV and its response to interferon therapy. Methods The TTV DNA in serum and liver tissue were detected by PCR and in situ hybridization. The virus and ALT response of 8 TTV / HCV infected patients after treatment with interferon were retrospectively analyzed. Results The prevalence of TTV in all kinds of liver diseases (36.7% -42.8%) and professional blood donors (43.2%) were significantly higher than those in voluntary donors (5.6% in healthy controls). TTV DNA (3/30, 10.0%) was detected in sera of some infected patients by dot blot hybridization, which indicated that there was a high serum viral load, 16 patients with chronic non-hepatitis E and hepatitis E and 16 after hepatitis Liver cirrhosis patients by in situ hybridization detection, 6 cases of TTV DNA positive (37.5%). Twenty-eight patients with TTV / HCV infection maintained long-term ALT response (normal ALT at 24 weeks after treatment) after 24 weeks of treatment with interferon. After 8 weeks of treatment, serum TTV DNA was negative in all 8 patients, but two Two patients with no response after ALT and ALT withdrawal had HCV RNA positive but negative TTV DNA, while two patients with TTV DNA positive ALT maintained response. Conclusion TTV infection is more common in liver disease patients and occupational blood donors. TTV can be actively replicated in liver tissue, which is more sensitive to interferon treatment. TTV / HCV infection does not induce liver injury obviously.