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目的通过对不同采收期大青叶质量的差异进行比较,确定大青叶合理的采收期。方法采用高效液相色谱法对不同采收期大青叶中靛玉红含量进行测定分析;采用火焰原子吸收分光光度法对大青叶中包括3种重金属在内的8种无机元素进行了测定分析。结果7月采收的药材中靛玉红的含量明显高于11月采收药材。7月采收大青叶中Ca、K、Zn、Cu元素的含量均高于11月;11月采收药材中Pb等3种重金属含量均大于7月采收药材。结论为确保临床用药的安全有效,有必要调整大青叶传统采收时间,应以7月为最佳采收季节。
OBJECTIVE To compare the differences of the quality of Folium Isatidis in different harvest periods and determine the reasonable harvest period of Folium Isatidis. Methods High performance liquid chromatography (HPLC) was used to determine the content of indirubin in the leaves of Folium Isatidis. Eight kinds of inorganic elements including the three heavy metals in Folium Isatidis were determined by flame atomic absorption spectrometry analysis. Results The content of indirubin collected in July was significantly higher than that of the harvest in November. The contents of Ca, K, Zn and Cu in the Folium Isatidis collected in July were all higher than those in November. The contents of Pb and other heavy metals in the collected medicinal materials in July were all higher than those harvested in July. Conclusion In order to ensure the safety and effectiveness of clinical medication, it is necessary to adjust the traditional harvest time of Folium Isatidis, and July should be the best harvesting season.