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目的:优选石韦总黄酮的大孔树脂纯化工艺条件,为该成分的制剂开发提供参考。方法:采用紫外-可见分光光度法测定总黄酮含量,检测波长510 nm。以总黄酮含量为指标,通过静态吸附-洗脱试验筛选大孔树脂型号,利用单因素试验考察上样量、径高比、洗脱剂用量等因素对石韦总黄酮大孔树脂纯化工艺的影响。结果:D101型大孔树脂吸附-洗脱性能最好,最佳纯化工艺条件为树脂柱径高比1∶10~1∶8,树脂-生药(1∶2.73),上样液质量浓度0.5 g·m L-1,上样速度2 BV·h-1,加水7 BV洗脱,加60%乙醇5 BV以流速4 BV·h-1洗脱。石韦总黄酮纯度达59.06%。结论:D101型大孔树脂对石韦总黄酮具有良好的纯化效果,优选的纯化工艺合理、稳定可行。
Objective: To optimize the macroporous resin purification conditions of total flavonoids, and provide a reference for the formulation development of this component. Methods: The content of total flavonoids was determined by UV - Vis spectrophotometry. The detection wavelength was 510 nm. The total flavonoids content as an indicator, through the static adsorption - elution test screen macroporous resin model, the use of single factor test sample size, diameter and height ratio, the amount of eluent and other factors on the total flavonoids Secreta macroporous resin purification process influences. Results: The best adsorption and elution properties of D101 macroporous resin were as follows: the resin column diameter-height ratio 1:10 ~ 1: 8, the resin-crude drug (1:2.73), the sample mass concentration 0.5 g · ML-1, loading rate 2 BV · h-1, water 7 BV, and 60% ethanol 5 BV at a flow rate of 4 BV · h-1. Shiwei total flavonoids purity of 59.06%. Conclusion: The D101 macroporous resin has a good purification effect on the total flavonoids of Chuanwei, and the optimized purification process is reasonable and stable.