【摘 要】
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Paraquat is one of the most widely used nonselective herbicides and has elicited the emergence of para-quat-resistant weeds.However,the molecular mechanisms of paraquat resistance are not completely un-derstood.Here we report the Arabidopsis gain-of-funct
【机 构】
：
School of Life Sciences,Division of Molecular & Cell Biophysics,Hefei National Science Center for Ph
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Paraquat is one of the most widely used nonselective herbicides and has elicited the emergence of para-quat-resistant weeds.However,the molecular mechanisms of paraquat resistance are not completely un-derstood.Here we report the Arabidopsis gain-of-function mutant pqt15-D with significantly enhanced resistance to paraquat and the corresponding gene PQT15,which encodes the Multidrug and Toxic Extru-sion (MATE) transporter DTX6.A point mutation at +932 bp in DTX6 causes a G311E amino acid substitution,enhancing the paraquat resistance ofpqt15-D,and overexpression of DTX6/PQT15 in the wild-type plants also results in strong paraquat resistance.Moreover,heterologous expression of DTX6 and DTX6-D in Es-cherichia coil significantly enhances bacterial resistance to paraquat.Importantly,overexpression of DTX6-D enables Arabidopsis plants to tolerate 4 mM paraquat,a near-commercial application level.DTX6/PQT15 is localized in the plasma membrane and endomembrane,and functions as a paraquat efflux transporter as demonstrated by paraquat efflux assays with isolated protoplasts and bacterial cells.Taken together,our results demonstrate that DTX6/PQT15 is an efflux transporter that confers paraquat resis-tance by exporting paraquat out of the cytosol.These findings reveal a molecular mechanism of paraquat resistance in higher plants and provide a promising candidate gene for engineering paraquat-resistant crops.
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