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为了揭示云南栽培辣木的遗传多样性和亲缘关系,为国内辣木种质资源的进一步有效开发与利用提供依据,采用AFLP技术对27份云南栽培辣木材料进行了遗传多样性分析,筛选得到9对条带清晰、多态性高的引物,共扩增出946条条带;其中,多态性条带913条,平均每对引物扩增条带105条,多态性条带101条,多态性位点频率为96.19%,表明云南栽培辣木具有较为丰富的遗传多态性。27份材料间遗传相似系数变化范围为0.657 5~0.838 3。UPGMA聚类结果表明:27份材料可分为6类,来自同一栽培区域的材料并没有聚在一起,具有相同性状的材料也没有聚在一起,表明栽培辣木种群内遗传分化较大,遗传背景复杂,相关性状和遗传的关系仍需进一步研究。
In order to reveal the genetic diversity and genetic relationship of cultivated Moringa in Yunnan and provide the basis for further effective development and utilization of Moringa germplasm resources in China, AFLP was used to analyze the genetic diversity of 27 cultivated Moringa cultivars in Yunnan Province. Nine 946 bands were amplified for the primers with clear bands and high polymorphism. Among them, 913 bands were polymorphic, with 105 bands amplified per primer pair and 101 bands , The frequency of polymorphic loci was 96.19%, indicating that Yunnan cultivation Moringa has a rich genetic polymorphism. The genetic similarity coefficients of 27 materials varied from 0.657 5 to 0.838 3. UPGMA clustering results showed that: 27 materials can be divided into 6 categories, materials from the same cultivation area did not get together, and materials with the same traits also did not get together, indicating that there was a large genetic differentiation in the cultivated Moringa population, The background is complex, the relationship between traits and genetic needs further study.