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目的:建立东风桔总生物碱的含量测定方法并优选其提取工艺。方法:采用酸性染料比色法测定东风桔总生物碱含量,选择Buxifoliadine-B为指标成分,检测波长410 nm。以总生物碱提取量为指标,通过正交试验考察乙醇体积分数、乙醇用量、回流时间及提取次数对提取工艺的影响。结果:Buxifoliadine-B含量测定的线性范围0.034~0.34 g·L-1,加样回收率(99.16±1.81)%。最佳提取工艺为加10倍量80%乙醇回流提取2次,每次1.5 h;总生物碱提取量4.88 mg·g-1。结论:建立的东风桔总生物碱含量测定方法稳定可靠,优选的提取工艺方法合理可行。
OBJECTIVE: To establish a method for the determination of total alkaloids in Dongfeng orange and optimize its extraction process. Methods: The content of total alkaloids in P. orientalis was determined by acid dye colorimetric method. Buxifoliadine-B was selected as the index component and the detection wavelength was 410 nm. Taking total alkaloid extract as an index, the effects of ethanol volume fraction, ethanol dosage, reflux time and extraction times on the extraction process were investigated by orthogonal test. Results: The linear range of determination of Buxifoliadine-B was 0.034-0.34 g · L -1, and the recovery rate was (99.16 ± 1.81)%. The best extraction process for the extraction of 10 times the amount of 80% ethanol was extracted twice, 1.5 h each; total alkaloid extract 4.88 mg · g-1. Conclusion: The established method for determination of total alkaloids in Dongfeng orange is stable and reliable, and the optimal extraction process is reasonable and feasible.