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目的:探讨芪防鼻敏颗粒对变应性鼻炎(AR)大鼠行为学、鼻腔分泌物嗜酸性粒细胞(EOS)及鼻黏膜组织病理学的影响。方法:SD大鼠随机分为空白对照组、模型组、氯雷他定组(1.7 mg·kg~(-1))、鼻炎康组(0.6 g·kg~(-1))和芪防鼻敏颗粒高(4.6 g·kg~(-1))、中(2.3 g·kg~(-1)),低(1.15 g·kg~(-1))剂量组。采用卵清蛋白(OVA)制备AR大鼠模型,模型建立成功后,灌胃给药,每日1次,连续14 d。对各组大鼠行为学症状进行评分,检测鼻腔分泌物EOS含量,观察鼻黏膜组织病理学变化。结果:与模型组相比,芪防鼻敏颗粒各剂量组行为学评分明显降低(P<0.01),鼻腔分泌物EOS浸润明显下降(P<0.05或P<0.01)。组织病理学结果显示,芪防鼻敏颗粒各剂量组黏膜上皮基本完好,部分可见腺体增生,血管轻度充血,少量EOS浸润,与模型组比较,病理评分显著降低(P<0.05或P<0.01)。结论:芪防鼻敏颗粒可降低AR大鼠行为学评分,减轻鼻腔分泌物EOS浸润,改善大鼠鼻黏膜组织病理学,对AR大鼠起到一定的治疗作用。
Objective: To investigate the effect of Qifangbimin Granules on behavior, eosinophilic nasal secretions (EOS) and histopathology of nasal mucosa in rats with allergic rhinitis (AR). Methods: SD rats were randomly divided into blank control group, model group, loratadine group (1.7 mg · kg -1), rhinitis group (0.6 g · kg -1) Sensitive granules were high (4.6 g · kg -1), medium (2.3 g · kg -1) and low (1.15 g · kg -1). The rat model of AR was established by ovalbumin (OVA). After successful establishment of the model, the rats were given gavage once a day for 14 days. The behavioral symptoms of rats in each group were scored, the content of nasal secretions EOS was measured, histopathological changes of nasal mucosa were observed. Results: Compared with the model group, the Qifangbimin Granules significantly decreased the scores of behavior (P <0.01) and the nasal secretions EOS infiltration decreased significantly (P <0.05 or P <0.01). Histopathological results showed that the mucosal epithelium of each dose group of Qifangbimin Granules was basically intact, with gland hyperplasia, slight blood vessel hyperemia, and a small amount of EOS infiltration. Compared with the model group, the pathological score was significantly lower (P <0.05 or P < 0.01). Conclusion: Qifangbimin Granules can reduce the behavioral score of AR rats, relieve nasal secretions EOS infiltration, improve the histopathology of nasal mucosa in rats and play a therapeutic role in AR rats.