论文部分内容阅读
【目的】建立鸡蛋花环烯醚萜含量测定方法,优选其提取工艺。【方法】采用紫外分光光度法,以梓醇为对照品,经2,4-二硝基苯肼显色后,在463 nm处测定其含量,并利用单因素结合正交设计优选最佳的提取工艺。【结果】该显色方法稳定,梓醇浓度在0.0220~0.0882 mg/m L范围内线性关系良好,回归方程为Y=10.18 X-0.020,R2=0.999,平均回收率为99.72%,相对标准偏差sR=2.9%(N=6)。优化的工艺条件是乙醇浓度60%(体积分数),液料比25∶1,超声提取60 min。【结论】该方法准确,简单,稳定性良好,工艺稳定可行,可为鸡蛋花生产工艺及质量控制提供参考。
【Objective】 The objective of this study was to establish a method for the determination of the iridoid content of the egg cane and optimize its extraction process. 【Method】 UV-Vis spectrophotometry was used to determine the content of catalpol at 463 nm after 2,4-dinitrophenylhydrazine was developed. The optimal combination of single factor and orthogonal design Extraction process. 【Result】 The method was stable. The linear range of catalpol concentration was 0.0220 ~ 0.0882 mg / m L. The regression equation was Y = 10.18 X-0.020, R2 = 0.999, the average recovery was 99.72%. The relative standard deviation sR = 2.9% (N = 6). The optimum conditions were ethanol concentration 60% (volume fraction), liquid to material ratio 25: 1, ultrasonic extraction 60 min. 【Conclusion】 The method is accurate, simple and stable, and the process is stable and feasible. It can provide reference for the production technology and quality control of egg flower.