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本研究以5份转基因大豆株系和2份非转基因大豆株系的种子为试验材料,设置4种盐浓度,采用砂培的方法在实验室进行标准发芽试验,观察不同株系在不同盐浓度条件下萌发和苗期的生长情况,鉴定不同株系耐盐性差异,筛选耐盐株系;取转基因植株幼嫩叶片进行基因组DNA的提取,然后利用PCR技术检测转化的耐盐目的基因是否存在。本研究取得如下研究结果:在相同温度条件下,转基因株系的发芽率均高于未转入目的基因的对照品种,其中BADH-14、BADH-17、CMO-5的耐盐性明显高于对照吉农17。
In this study, the seeds of 5 transgenic soybean lines and 2 non-transgenic soybean lines were used as test materials, four kinds of salt concentration were set, and the standard germination test was carried out in the laboratory by sand culture method. The effects of different salt concentrations Germination and seedling growth conditions, identify different strains of salt tolerance differences, screening salt-tolerant strains; young leaves of transgenic plants for genomic DNA extraction, and then use PCR to detect the presence of transformed salt tolerance genes . The results of this study are as follows: Under the same temperature conditions, the germination rate of the transgenic lines were higher than the control varieties not transferred into the target gene, of which BADH-14, BADH-17, CMO-5 salt tolerance was significantly higher Control of jinong 17.