血浆中的ACTH、β-LPH、β-内啡肽和甲硫-脑啡肽用放射免疫法测定,血糖用Neocuproine法测定,皮质醇用荧光法测定。以6个正常人为实验对象,在用胰岛素引起降血糖的同时,血浆中ACTH、β-LPH和β-内啡肽的免疫活性升高,但甲硫-脑啡肽没有明显的变化。在反应高峰(45分钟)时取血浆样品经Sephadex G75层析,进一步 Plasma ACTH, β-LPH, β-endorphin and methionine-enkephalin were measured by radioimmunoassay, blood glucose was measured by Neocuproine method, and cortisol was measured by fluorimetry. Six normal subjects were used to induce hypoglycaemic hypoglycemia, while the immunological activities of ACTH, β-LPH and β-endorphin in plasma were increased, but there was no obvious change in methione-enkephalin. Plasma samples were taken by Sephadex G75 chromatography at the peak of the reaction (45 minutes) and further