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以259个小麦微核心种质为材料进行剂量为0、100、150、250 Gy的60Coγ射线辐照处理,探讨小麦微核心种质的γ射线辐射敏感性分布,以及DNA损伤修复基因TaKu70和TaKu80对辐照的应答模式。结果表明,小麦微核心种质的苗高损伤率与γ射线辐照剂量间存在着3种函数关系:对数、线性、幂函数。以苗高损伤率为50%时的辐照剂量HD50作为主要的辐射敏感性分型指标,分别统计不同函数关系的微核心种质落入不同剂量区间的基因型个数,并依此将259份微核心种质分为敏感型(10)、较敏感型(96)、较钝感型(101)、钝感型(52)。对数函数关系中以敏感型和较敏感型为主,随着γ射线辐照剂量的增加TaKu70和TaKu80基因的相对表达量与对照相比总体升高,但变化不明显;线性函数关系中以较敏感型和较钝感型为主,TaKu70和TaKu80基因的相对表达量与对照相比总体升高,随剂量的增加而逐渐递增;幂函数关系中以较钝感型和钝感型为主,TaKu70和TaKu80基因的相对表达量与对照相比总体升高,但随剂量的增加呈现先增加后降低的趋势,一般相对表达量的峰值出现在150 Gy。
A total of 259 wheat micro-core germplasms were irradiated with 60Co γ-rays at doses of 0, 100, 150 and 250 Gy to investigate the γ-ray radiation-sensitive distribution of wheat micro-core germplasm, and the DNA damage repair genes TaKu70 and TaKu80 Response mode to radiation. The results showed that there were three kinds of functional relationship between the seedling height damage rate and γ-ray irradiation dose of wheat micro-core germplasm: logarithm, linearity and power function. The radiation dose HD50 was used as the main radiation sensitivity classification index when the damage rate was 50%, and the numbers of genotypes with different kernels of micro-kernels falling into different dose ranges were calculated respectively. Based on this, 259 The micro-core germplasms were classified as sensitive type (10), sensitive type (96), more obtuse type (101) and insensitive type (52). The relationship between logarithmic function is sensitive and sensitive. The relative expression of TaKu70 and TaKu80 gene increased with the increase of γ-ray dose, but the change was not obvious. In the linear function, The more sensitive and the more obtrusive, the relative expression of TaKu70 and TaKu80 genes increased as compared with the control, and gradually increased with the increase of dosage; the more obtrusive and insensitive The relative expression of TaKu70 and TaKu80 genes increased as compared with the control. However, the relative expression of TaKu70 and TaKu80 increased first and then decreased with the increase of the dose.