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目的探讨木犀草素对自发性高血压大鼠(SHR)心肌组织中醛糖还原酶(AR)活性及mRNA表达的影响及作用机制。方法选用50只18周龄SHR,随机均分为SHR组(模型阴性对照组)、卡托普利组、木犀草素10、20、40 mg.kg-1.d-1剂量组、WKY大鼠组(正常对照组)。观察AR、血管紧张素Ⅱ(AngⅡ)的变化;以RT-PCR法检测木犀草素对SHR心肌组织AR的mRNA表达。结果木犀草素各剂量组与模型组比较:心肌AR活性和AngⅡ活性均下降(P<0.05),心肌AR的mRNA水平下调(P<0.05),各组AR活性变化趋势与AngⅡ浓度变化成直线相关性(r=0.9686,P<0.05)。结论木犀草素可显著抑制AR的活性,其机制可能与下调mRNA表达及抑制AngⅡ有关。
Objective To investigate the effect and mechanism of luteolin on the activity of aldose reductase (AR) and mRNA expression in myocardium of spontaneously hypertensive rats (SHR). Methods Fifty 18-week-old SHRs were randomly divided into SHR group (model negative control group), captopril group, luteolin (10,20,40 mg.kg-1.d-1), WKY group Rat group (normal control group). The changes of AR and Ang Ⅱ were observed. The mRNA expression of AR was detected by RT-PCR in SHR myocardium. Results Compared with the model group, the luteolin content in each dose group decreased: AR activity and AngⅡ activity in myocardium decreased (P <0.05), AR mRNA expression in myocardium decreased (P <0.05), and the trend of AR activity in each group changed linearly with Ang Ⅱ concentration Correlation (r = 0.9686, P <0.05). Conclusion Luteolin can significantly inhibit the activity of AR, and its mechanism may be related to the down-regulation of mRNA expression and the inhibition of AngⅡ.