[目的]探讨结肠癌转移相关基因1(MACC1)和KAI1／CD82 蛋白在肝细胞癌(HCC)组织中的表达情况及对患者生存情况的影响.[方法]采用免疫组织化学法检测本院病理科收集的 61 例 HCC患者癌组织以及癌旁肝组织中 MACC1 和KAI1／CD82 蛋白表达情况,比较两组间的 MACC1 和 KAI1／CD82 蛋白表达差异.[结果]与癌旁组织相比,HCC癌组织中 MACC1 蛋白表达水平显著增加(P＜0.05),而 KAI1／CD82蛋白表达水平显著降低(P＜0.05).MACC1 和 KAI1／CD82 蛋白水平与肿瘤分级、淋巴结转移、侵犯深度、TNM分期相关(P＜0.05).MACC1 阴性或KAI1／CD82 阳性 HCC患者总体生存时间显著高于 MACC1 阳性或KAI1 阴性 HCC患者(P＜0.05).多因素分析显示,MACC1、KAI1／CD82 阳性表达以及 TNM分期均为影响患者生存时间的因素(P＜0.05).[结论]MACC1 和KAI1／CD82 表达异常在 HCC发展中发挥重要作用,MACC1 和KAI1／CD82 联合检测是 HCC患者潜在的有效判别肿瘤转移和预后的生物标志物.“,”[Objective]To explore the expression of MACC1 and KAI1／CD82 in hepatocellular carcinoma (HCC)patients ,as well as the correlation with clinicopathological features.[Methods]The expression levels of both MACC1 and KAI1／CD82 in 61 cases of HCC tissues and corresponding adjacent liver tissues were detected by immunohistochemistry,and the differences of MACC1 and KAI1／CD82 protein expression between the two groups were compared.According to the clinical and pathological typing,the specimens of HCC radi cal resection group were grouped in order to reveal the difference of expression between the groups,and further explore the correlation between the expression of MACC1 and KAI1／CD82 protein and clinical features.[Results]Compared to adjacent tissues,MACC1 was significantly overexpressed in HCC tissues (P ＜0.05), while KAI1／CD82 was significantly downexpressed (P ＜0.05).The levels of MACC1 and KAI1／CD82 protein were correlated with tumor grade,lymph node metastasis,depth of invasion,and TNM stage (P ＜0.05).The overall survival time of patients with MACC1or KAI1／CD82positive HCC tumors was signifi cantly longer than that of patients with MACC1 positive or KAI1 negative HCC (P ＜0.05).Importantly, multivariate analysis suggested that positive expression of either MACC1 or KAI1／CD82,as well as TNM stage,could be independent prognostic factors for overall survival in patients with HCC.[Conclusion]Abnormal expression of MACC1 and KAI1／CD82 plays an important role in the development of HCC.The combined detection of MACC1 and KAI1／CD82 is a biomarker for potential differential diagnosis of tumor metastasis and prognosis in HCC patients.MACC1 and KAI1／CD82 may represent promising metastatic and prognostic biomarkers,as well as potential therapeutic targets,for HCC.