论文部分内容阅读
目的研究氨基酸碘杀菌效果及其作用机理,为其应用于实践提供依据。方法采用悬液定量杀菌试验,透射电镜和生化检测等方法进行了实验室观察。结果含有效碘200mg/L的氨基酸碘溶液作用3~20min,对悬液内白色念珠菌的平均杀灭率范围为99.91%~99.96%;含有效碘400mg/L该消毒液作用3min,对白色念珠菌的杀灭率可达到100%。经氨基酸碘处理的白色念珠菌外层质膜电子密度明显降低,继而菌体形态可出现轻度不规则,细胞质中细胞器和核质结构形态混乱。氨基酸碘作用可使白色念珠菌膜蛋白含量减少,细菌碱性磷酸酶和琥珀酸脱氢酶渗出,且琥珀酸脱氢酶活性有所抑制;细菌核酸无降解发生,但有少量交联物生成。结论氨基酸碘对白色念珠菌杀灭作用较强。氨基酸碘可使细菌膜通透性明显增加,膜结构蛋白降解,胞质及蛋白酶渗出,琥珀酸脱氢酶活性显著降低,少量细菌核酸发生交联反应。
Objective To study the bactericidal effect of amino acid iodine and its mechanism of action and provide the basis for its application in practice. Methods Suspension quantitative germicidal test, transmission electron microscopy and biochemical tests and other methods were observed in the laboratory. Results The effective iodine 200mg / L amino acid iodine solution for 3 ~ 20min, the average kill rate of Candida albicans in the suspension ranged from 99.91% to 99.96%; effective iodine 400mg / L the disinfectant for 3min, white Candida kill rate up to 100%. The electron density of the outer membrane of Candida albicans treated with amino acid iodine decreased significantly, and then the morphology of the cells was slightly irregular. The structure of the organelles and nucleoplasm in the cytoplasm was confused. Amino acid iodine can reduce Candida albicans membrane protein content, bacterial alkaline phosphatase and succinate dehydrogenase exudation, and inhibition of succinate dehydrogenase activity; no degradation of bacterial nucleic acid, but a small amount of cross-linked generate. Conclusion Amino acid iodine has a strong killing effect on Candida albicans. Amino acid iodine can significantly increase bacterial membrane permeability, membrane protein degradation, exocytosis and protease exudation, succinate dehydrogenase activity was significantly reduced, a small amount of bacterial nucleic acid cross-linking reaction.